AssayMAP® Protein A Cartridges (PA Cartridges)

an open platform to purify and quantitate microscale samples

Protein A is widely used to purify therapeutic antibodies during cell culture process development. Purification is the required first step for both quantitation and characterization of the antibody product. The current “gold standard” method for quantitation – protein A affinity HPLC – is reliable, selective, sensitive and precise, but throughput is limited. Other methods are either complex to perform or require dedicated instrumentation. AssayMAP® Protein A Cartridges were developed to bring this industry standard protein A affinity purification chemistry to an open, microscale platform, delivering dramatically improved throughput by means of parallel sample processing. AssayMAP PA cartridges enable purification for both quantitation and analysis at the same time.

The heart of the technology is the AssayMAP cartridge containing a 5 µL bed packed with a rapid mass transport protein A affinity resin. These highly versatile cartridges can be operated as parallel ultra-micro spin columns using standard laboratory pipetting equipment and plate centrifuges or highly parallel, precision flow columns using 96-channel probe syringe technology and automated liquid handling. Controlled flow provided by specially engineered labware enables precise, quantitative binding and elution. The cartridges are reusable making them simple to use, efficient and cost effective.

What’s the advantage? 100-fold increase in throughput using standard laboratory equipment and your current validated reagents. All you need to get started is the AssayMAP PA50 Starter Kit, a microplate centrifuge, your current HPLC buffers, a micropipettor, plate reader, and the basic protocol. The same workflow also can be fully automated on conventional robotic liquid handlers.

snapshot protocol

storage and use conditions

Store unused cartridges at 4°C and use within 6 months of purchase.

IgG Standard Curve on AssayMAP PA50 Cartridges

A 2x serial dilution of hIgG was made from 5000 µg/mL downward, and 10, 30 and 100 µL samples of each standard were run. Readout was done using A₂₈₀. Recovery is highly linear up to approximately 100 µg hIgG, then flattens out due to saturation of the cartridge binding capacity.

Resource Library

• Guidebook — AssayMAP Spin Format Technology
• AssayMAP Protein A Cartridges protocol (PDF)

AssayMAP PA50 cartridges are made with POROS™ MabCapture™ A media from Life Technologies Corporation. POROS™ and MabCapture™ are Registered Trademarks of Life Technologies Corporation.