Product Concentration
AssayMAP cartridges provide true chromatography capabilities in a high-throughput, miniaturized format from sample volumes ranging from 5 to 200 µL through binding and elution while allowing highly precise control of volume and flow rate to quantitative results. Use these cartridges for microliter-scale separation and sample preparation with analytical instruments including plate readers, mass spectrometers and LC/MS systems. “Open chemistry” supports a broad range of applications from bind-and-elute assays, to ELISAs, to enzymatic digestions, to complex, multi-step sample preparation workflows.
AssayMAP cartridges can be filled with virtually any chromatographic resin or other beaded matrix:
- Immobilized antibodies
- Affinity ligands (such as streptavidin, protein A/G, lectins or IMAC)
- Selective adsorbent materials (such as ion exchange or reversed phase)
- Size exclusion & desalting
- Immobilized enzymes (such as proteases or glycosidases)
A typical application might include highly selective purification, enzymatic digestion and reversed-phase concentration/desalting steps of the resulting peptides in preparation for MS. These different steps can be integrated on the AssayMAP platform into a single automated workflow, enabling multiple analytes from complex samples (such as cell culture supernatant or plasma) to be determined in a single run. AssayMAP workflows may include multiple wet chemical assays, ELISAs and LC/MS analysis, all run on the same samples on the same system.
Learn more about our Protein A affinity toolbox: