Modular Chemistries

Modern biomolecule assays involve more than the simple addition of reagents. Protocols often incorporate highly selective purification methods, require quantitation and can integrate enzymatic reactions to prepare complex samples for downstream analysis on instruments such as plate readers, HPLC, LC/MS or CE. Purification methods range from physical techniques such as ion exchange chromatography to bioaffinity methods utilizing antibodies, affinity ligands, tags, receptors and more.

An open platform to purify, quantitate & react microscale samples

AssayMAP® microchromatography technology was developed to meet the needs of the most critical workflows that require chromatographic separations for microscale protein samples and demand a high-throughput, high-precision solution. The heart of the AssayMAP technology is a 5 µL, disposable, microchromatography cartridge packed with media to selectively purify, quantitate and/or react your target proteins for direct assay or downstream processing. AssayMAP cartridges can be used as modular building blocks, assembled in a wide variety of ways to create both simple and complex assay workflows for a wide range of applications.

Purification employs selective binding of a target,
washing to remove non-binding impurities and
elution to recover the bound target. AssayMAP
cartridges allow quantitative binding and elution.

 

Quantitation employs the same workflow as
purification and allows a precise, accurate
measurement of target concentration in a
microplate well by standard plate-based methods.

 

After immobilization of target biomolecule on a
cartridge (and optional blocking of vacant binding
sites), a controlled-temperature enzyme reaction
can be performed before selective elution of
release products and treated target.